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Studies on Bioconjugation of Vitamin B12 and its Application in Biosensor

Dhande, Sachin Dnyandeo (2010) Studies on Bioconjugation of Vitamin B12 and its Application in Biosensor. [Student Project Report]

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This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type: Student Project Report
Additional Information: Vitamin B12 analysis is targeted now a days due to its complex nature but vital role in nutrition and malfunctioning of the body. Due to complexity of structure and interferences produced by analogues demands specific methods for its determination. For achieving this determination more rapid methods are needed to be developed. These methods can be made very much handful by using bioconjugates. Bioconjugation is a burgeoning field of research. Novel methods for the bioconjugations of compounds with ligands had been developed for analysis purpose. These methods owe their existence to the discovery of chemoselective reactions that enable bioconjugation under physiological conditions—a tremendous achievement of modern organic chemistry. Here, in this project a novel bioconjugation of vitamin B12 with ligand such as alkaline phosphatase was carried out and optimized. We have done study of percentage conjugation in conjugate , confirmation was done with HPLC and TNBS assay.
Uncontrolled Keywords: vitamin B12, vitamin B12 conjugates, Biosensor
Subjects: 600 Technology > 05 Chemical engineering > 01 Biotechnology and Bioengineering
600 Technology > 08 Food technology > 16 Nutritive value > 04 Vitamins
Divisions: Fermentation Technology and Bioengineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 30 Nov 2010 05:37
Last Modified: 28 Dec 2011 10:19
URI: http://ir.cftri.com/id/eprint/9802

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