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Inhibition of human platelet aggregation and membrane lipid peroxidation by food spice, saffron.

Jessie Suneetha, W. and Krishnakantha, T. P. (2005) Inhibition of human platelet aggregation and membrane lipid peroxidation by food spice, saffron. Molecular and Cellular Biochemistry, 278 (1-2). pp. 59-63. ISSN 0300-8177

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Abstract

The inhibitory activity of saffron extract was studied on human platelets. Platelet aggregation and lipid peroxidation were evaluated with platelet rich plasma (PRP) and platelet membranes respectively obtained from blood of healthy human volunteers. Human platelets were subjected to stimulation with a variety of agonists like ADP (61 microM), epinephrine (76 microM), collagen (11 microg/ml), calcium ionophore A 23187 (6 microM) and ristocetin (1.25 microg/ml) in the presence and absence of saffron extract with IC50 being 0.66, 0.35, 0.86 and 0.59 mg respectively and no inhibition with ristocetin. The inhibitory effect was dose dependent with concentrations varying between 0.16 to 0.80 mg and time dependent at IC50. A significant decrease was observed in malondialdehyde (MDA) formed, one of the end products of arachidonic acid metabolism and of serotonin released from dense granules of platelets at respective IC50. Lipid peroxidation in platelet membranes induced by iron-ascorbic acid system was inhibited by saffron extract significantly with IC50 of 0.33 mg. Hence, it may be said that aqueous extract of saffron may have component(s), which protect platelets from aggregation and lipid peroxidation.

Item Type: Article
Uncontrolled Keywords: human platelets, lipid peroxidation, malondialdehyde, platelet membranes, platelet aggregation, saffron, serotonin
Subjects: 600 Technology > 08 Food technology > 30 Spices/Condiments
500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 11 Lipid Biochemistry
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 02 Jul 2009 09:42
Last Modified: 14 Dec 2016 10:12
URI: http://ir.cftri.com/id/eprint/1974

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