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The RNA polymerase I subunit Rpa12p interacts with the stress-responsive transcription factor Msn4p to regulate lipid metabolism in budding yeast.

Kamlesh Kumar, Yadav and Neelima, Singh and Praveen Kumar, Rajvanshi and Ram, Rajasekharan (2016) The RNA polymerase I subunit Rpa12p interacts with the stress-responsive transcription factor Msn4p to regulate lipid metabolism in budding yeast. FEBS letters, 590. pp. 3559-3573. ISSN 0014-5793

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Abstract

In Saccharomyces cerevisiae, RPA12 encodes the small subunit of RNA polymerase I. Here, we demonstrate that Rpa12p interacts with the transcription factor Msn4p and prevents its binding to the promoter of AYR1 encoding Ayr1p (1-acyldihydroxyacetone phosphate reductase), a key enzyme involved in triacylglycerol biosynthesis and mobilization of nonpolar lipids. Deletion of RPA12 leads to triacylglycerol accumulation due to the binding of Msn4p to the promoter of AYR1 and activation of its transcription. The double deletion rpa12D::ayr1D caused a reduction in triacylglycerol levels. Our findings reveal that Rpa12p functions as a negative regulator of lipid metabolism by modulating nonpolar lipid biosynthesis through its interaction with Msn4p.

Item Type: Article
Uncontrolled Keywords: nonpolar lipid metabolism; transcriptional regulation; triacylglycerol
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology > 11 Lipid Biochemistry
600 Technology > 01 Medical sciences > 12 Metabolism
600 Technology > 08 Food technology > 29 Microbiological food > 04 Yeast
Divisions: Dept. of Lipid Science
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 31 May 2019 06:22
Last Modified: 31 May 2019 06:22
URI: http://ir.cftri.com/id/eprint/14103

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