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Expression profiling of the Dolichos lablab lectin during germination and development of the seed.

Yashavanth, L. Vishweshwaraiah and Balaji, Prakash and Lalitha, R. Gowda (2018) Expression profiling of the Dolichos lablab lectin during germination and development of the seed. Plant Physiology and Biochemistry, 124. pp. 10-19.

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Abstract

The temporal expression of the field bean (Dolichos lablab) galactose specific lectin, DLL-II, during germination, post-germination and seed development was evaluated using Native-PAGE followed by activity staining, immunodetection and quantitative Real Time PCR (qPCR). A rapid and steep decline in the polyphenol oxidase (PPO) and hemagglutinating activity during the initial stages of germination, which did not correlate with the slow decline in total protein was observed. During post germination period, PPO and hemagglutination activities were negligible, whereas a rapid resorption of the protein was evident. These results suggest that DLL-II is not a storage protein. The presence of mRNA in the quiescent seed and initial stages of germination are indicative of a very stable mRNA. DLL-II was expressed in high copies during seed development and increased dramatically between 10 - 20 days after flowering (DAF), suggesting a switch over stage in DLL-II expression. Transcript levels reached a maximum at the mature stage of seed development. Among the non-seed tissues examined, root showed the highest level. The high affinity binding to kinetin and indole acetic acid, the key hormones that regulate root development and its vascular differentiation add a new dimension to the physiological role of DLL-II in the seed. This finding, coupled with the PPO and hemagglutinating activity makes DLL-II, a truly a multifunctional protein.

Item Type: Article
Uncontrolled Keywords: Defense proteins, phytohormone, storage protein, transcript, germination
Subjects: 600 Technology > 08 Food technology > 16 Nutritive value > 03 Proteins
600 Technology > 08 Food technology > 22 Legumes-Pulses
Divisions: Molecular Nutrition
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 12 Jul 2018 05:20
Last Modified: 12 Jul 2018 05:20
URI: http://ir.cftri.com/id/eprint/13590

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