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Single step aqueous two-phase extraction for downstream processing of C-phycocyanin from Spirulina platensis.

Chethana, S. and Chetan Nayak, A. and Madhusudhan, M. C. and Raghavarao, K. S. M. S. (2015) Single step aqueous two-phase extraction for downstream processing of C-phycocyanin from Spirulina platensis. Journal of Food Science and Technology, 52 (4). pp. 2415-2421.

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Abstract

C-phycocyanin, a natural food colorant, is gaining importance worldwide due to its several medical and pharmaceutical applications. In the present study, aqueous two-phase extraction was shown to be an attractive alternative for the downstream processing of C-phycocyanin from Spirulina platensis. By employing differential partitioning, Cphycocyanin selectively partitioned to the polymer rich (top) phase in concentrated form and contaminant proteins to the salt rich (bottom) phase. This resulted in an increase in the product purity (without losing much of the yield) in a single step without the need of multiple processing steps. Effect of process parameters such as molecular weight, tie line length, phase volume ratio, concentration of phase components on the partitioning behavior of C-phycocyanin was studied. The results were explained based on relative free volume of the phase systems. C-phycocyanin with a purity of 4.32 and yield of about 79 % was obtained at the standardized conditions.

Item Type: Article
Uncontrolled Keywords: Aqueous two-phase systems . C-phycocyanin . Relative free volume . Partition coefficient . Purification
Subjects: 600 Technology > 08 Food technology > 01 Analysis
600 Technology > 08 Food technology > 29 Microbiological food > 01 Algae
Divisions: Food Engineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 16 Jun 2015 08:15
Last Modified: 16 Jun 2015 08:15
URI: http://ir.cftri.com/id/eprint/11811

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