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“Production and Evaluation of Probiotic Lactic Acid Bacteria (LAB) for Therapeutic Application

GirishKumar, B. (2011) “Production and Evaluation of Probiotic Lactic Acid Bacteria (LAB) for Therapeutic Application. PhD thesis, University of Mysore.

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The thesis reports isolation, identification and characterization of probiotic lactic acid bacteria from healthy breast fed infants fecal matter and studies on the beneficial effects in vitro and in vivo models. The isolates were evaluated for potent probiotic properties by carrying out a number of in vitro studies. The selected isolates were characterized by phenotypic and genotypic methods. Out of 22 isolates two were selected as potent probiotic strains based on their in vitro performances. Both the selected isolates were identified as Lactobacillus fermentum. The isolates were designated as CFR 2195 and 2196. Lactobacillus fermentum CFR 2195 was selected for the further studies. Functional properties such as production of amino acids, vitamin B12, synbiotic antimicrobial effects and adherence towards HT-29 and Caco-2 cell-lines were carried out. A medium was designed with optimum physical and nutritional parameters for the efficient production of biomass and lactic acid. Productive performance in the optimum point medium, was closer to that of the MRS medium, however much cheaper when compared to MRS medium. There was no significant change in the quantity of biomass obtained from the optimized fermentation medium in comparison to that in MRS medium, while there was a considerable decrease (27.20%) in the cost. Thus, it could be said that the medium optimized in this study was not only economical but also favourable for the growth of L. fermentum. Probiotic L. fermentum CFR 2195 has shown anti-inflammatory activity in the intestines of TNBS model of rat colitis. The colonization of the probiotic L. fermentum in the colonic lumen resulted in positive effects, probably derived from their immunomodulatory properties. The administration of the probiotic L. fermentum CFR 2195 facilitates the recovery of the inflamed tissue in the TNBS model of rat colitis, an effect associated with amelioration of the production of some of the mediators involved in the inflammatory response of the intestine. This beneficial effect could be ascribed to its ability to prevent oxidative stress that occurs under inflammatory condition, through the increased production of glutathione, which might attenuate the exacerbated immune response evoked by the colonic instillation of the hapten TNBS in the rats.

Item Type: Thesis (PhD)
Uncontrolled Keywords: lactic acid bacteria, probiotic properties, L. fermentum, therapeutic application
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology
Divisions: Fermentation Technology and Bioengineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 14 May 2012 11:10
Last Modified: 18 Nov 2016 10:24
URI: http://ir.cftri.com/id/eprint/10746

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