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Agrobacterium rhizogenes-mediated transformation of Witloof chicory – In vitro shoot regeneration and induction of flowering

Harsh Pal, Bais and Venkatesh, R. T. and Chandrashekar, A. and Ravishankar, G. A. (2001) Agrobacterium rhizogenes-mediated transformation of Witloof chicory – In vitro shoot regeneration and induction of flowering. Current Science, 80 (1). pp. 83-87.

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Stable transformation and expression of transgene was achieved in Witloof chicory (Cichorium intybus L. cv. Lucknow local) using Agrobacterium rhizogenesmediated system. Transformation frequencies varied with use of different types of strains of A. rhizogenes, concentration of A. rhizogenes and age of explants. The transfer of rolA gene into chicory genome was confirmed by PCR analyses. The supplementation of putrescine at 40 mM level with 2¢-3¢-O-isopropylidene adenosine (2-iP) (2 mg l–1) and, gibberellic acid (GA3) (0.5 mg l–1) to MS medium resulted in increased shoot multiplication and shoot growth in untransformed plants compared to the transformed plants of C. intybus. Putrescine at 40 mM level also resulted in flowering in both the systems on the 28th day, in untransformed and transformed plants. The supplementation of AgNO3 at 40 μM level to the untransformed axillary buds of C. intybus on MS media with 2-iP (2 mg l–1) and GA3 (0.5 mg l–1) resulted in higher shoot multiplication (36.9 ± 2.63 shoots per culture) and higher growth of shoots (7.82 ± 0.76 cm) in comparison to transformed ones for shoot multiplication (11.6 ± 0.89 shoots per culture) and growth of shoots (3.2 ± 0.24 cm). Moreover, 40 μM AgNO3 treated cultures showed in vitro flowering on the 28th day in both the systems. The information generated in terms of transformation protocols, in vitro morphogenesis including flowering will make genetic transformation easier for chicory crop improvement.

Item Type: Article
Uncontrolled Keywords: A. rhizogenes, polymerase chain reaction (PCR) amplifications
Subjects: 600 Technology > 07 Beverage Technology > 04 Coffee
500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 06 Jun 2011 09:58
Last Modified: 28 Dec 2011 10:23
URI: http://ir.cftri.com/id/eprint/10069

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