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Process Assessment for the Purification of Aspergillus carbonarius Polygalacturonase Produced by Submerged and Solid-State Fermentations

Nakkeeran, E. and Umesh Kumar, S. and Subramanian, R. (2009) Process Assessment for the Purification of Aspergillus carbonarius Polygalacturonase Produced by Submerged and Solid-State Fermentations. International Journal of Engineering and Technology, 1 (4). pp. 278-281.

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Abstract

Abstract—Integrated membrane process (IMP) and alginate affinity precipitation (AAP) were assessed for the purification of Aspergillus carbonarius polygalacturonase (PG) obtained after submerged (SmF) and solid-state fermentations (SSF). IMP enhanced the purity (4.69 fold) and recovery (76%) of SmF-PG, but did not improve the purity of SSF and commercial PG due to the presence of other similar molecular mass proteins. AAP enhanced the PG purity and recovery in all the PG samples (SmF, SSF and commercial PG). IMP offers several process advantages over AAP but not suitable for purification of all types of PGs. Our studies on SmF- and SSF-PG had shown that downstream process needs are specific for individual cases and warrants careful considerations.

Item Type: Article
Uncontrolled Keywords: Alginate affinity precipitation, integrated membrane process, polygalacturonase, submerged and solid-state fermentations.
Subjects: 600 Technology > 05 Chemical engineering > 04 Fermentation Technology
500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology
Divisions: Food Microbiology
Food Engineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 24 Feb 2011 06:52
Last Modified: 28 Dec 2011 10:21
URI: http://ir.cftri.com/id/eprint/9929

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