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Aspergillus carbonarius polygalacturonases purified by integrated membrane process and affinity precipitation for apple juice production

Nakkeeran, Ekambaram and Umesh Kumar, S. and Subramanian, R. (2011) Aspergillus carbonarius polygalacturonases purified by integrated membrane process and affinity precipitation for apple juice production. Bioresource Technology , 102. pp. 3293-3297.

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Abstract

Aspergillus carbonarius, when grown by submerged and solid-state fermentation, produces different molecular forms of polygalacturonase (PG; EC 3.2.1.15), among them a 42 kDa PG with a high specific activity of 7000 U/mg protein. When the enzymes were purified by integrated membrane process (IMP) and alginate affinity precipitation (AAP), the two processes concentrated different forms of the enzyme. The AAP process selectively purified and concentrated the high active PG whereas the IMP yielded different PGs and also amylase and protease. Evaluation of the AAP enzyme preparations for apple juice preparation under conditions usually employed commercially demonstrated that the high activity PG did not result in good juice clarity. With IMP processed enzymes, juice yields and clarity were similar to that obtained with commercial PG from A. niger.

Item Type: Article
Uncontrolled Keywords: Aspergillus carbonarius Polygalacturonase Extraction Clarification Apple juice
Subjects: 600 Technology > 08 Food technology > 24 Fruits
500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology
600 Technology > 08 Food technology > 01 Analysis
Divisions: Food Engineering
Food Microbiology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 24 Feb 2011 06:48
Last Modified: 30 May 2012 11:14
URI: http://ir.cftri.com/id/eprint/9928

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