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Putrescine influences growth and production of coumarins in transformed and untransformed root cultures of witloof chicory (Cichorium intybus L. cv. Lucknow local)

Harsh Pal, Bais and Sudha, G. and Ravishankar, G. A. (2001) Putrescine influences growth and production of coumarins in transformed and untransformed root cultures of witloof chicory (Cichorium intybus L. cv. Lucknow local). Acta Physiologiae Plantarum, 23 (3). pp. 319-327.

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Abstract

The effect of putrescine on growth and production of two coumatins, esculin, and esculetin in the transformed and untranstbrmed roots of chicory (Cichorium intybus L. cv. Lucknow local) was examined. To study the role of putrescine (Put) on growth and production of coumarms, polyamine inhibitors namely a-DL-difluromethylornithine (DFMO) and a-L- difluromethylarginine (DFMA) were used at 1 mM levels. Treatment with 1.5 mM of putrescine (Put) produced 1.96 - fold and 4.0 - lbld increase in the growth of transformed and untransformed roots of chicory, respectively. The treatment with polyamine inhibitors showed much lower growth, as well as production compared with both 1.5 mM putrescine treatment and control in both transformed and untransformed chicory roots. The endogenous polyamines, both free and conjugated, were studied over the whole culture period, and it was seen that conjugated titers of all three polyamines viz., putrescine,spermidine and spermine were higher than level of free polyamines, throughout the culture period in both transformed and untransformed roots of chicory. Treatment in which polyamine inhibitors were used showed lower level of endogenous polyamines as compared with the 1.5 mM putrescine treated sample in both the systems. The treatment wherein putrescine was added at 1.5 mM level showed maximum accumulation of endogenous conjugated putrescine (2098.86 _+ 157.6 nmoles g-I FW; 896.8 _+ 67.2 nmoles.g d FW), on the 14 th day in both transformed and untransformed roots respectively. The production of esculin and esculetin was strictly correlated with growth in every treatment in both systems. Putrescine at 1.5 mM resulted in greater length of primary root in transformed (18.3 _+ 1.4 cm) and untransformed (6.86 _+ 0.51 cm) as compared with their respective controls (11 __+0_ .9 cm; 2.9 _+ 0. l cm) and greater number of secondary and tertiary roots. This study suggests that putrescine influences plant root development and differentiation, and it also provides insight into the morphological changes that occur in roots in response to the external supply of polyamines.

Item Type: Article
Uncontrolled Keywords: Cichorium intybus L. Cv. Lucknow local, transformed, untransformed, root cultures, polyamines, coumarins
Subjects: 600 Technology > 07 Beverage Technology > 04 Coffee
500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 15 Feb 2011 07:39
Last Modified: 19 Jun 2012 04:25
URI: http://ir.cftri.com/id/eprint/9916

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