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Physico-chemical Characterization of an Exopolysaccharide produced by a Non-ropy strain of Leuconostoc sp. CFR 2181 isolated from Dahi,an Indian Traditional Lactic fermented Milk Product

Vijayendra, S. V. N. and Palanivel, G. and Mahadevamma, S. and Tharanathan, R. N. (2008) Physico-chemical Characterization of an Exopolysaccharide produced by a Non-ropy strain of Leuconostoc sp. CFR 2181 isolated from Dahi,an Indian Traditional Lactic fermented Milk Product. Carbohydrate Polymers, 72. pp. 300-307.

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Abstract

Exopolysaccharide (EPS) producing non-ropy strain of lactic acid bacteria (LAB) was isolated from dahi, an Indian traditional fermented milk product, and on the basis of morphology, physiological and biochemical tests it was identified as Leuconostoc sp. CFR 2181. In shake flask fermentation for 72 h at 22 �C, the quantity of EPS produced by the isolate was 13.8 g/L in modified MRS broth and 25.4 g/L in EPS medium (a newly formulated simplified synthetic medium). The EPS was non-gelling and non-film forming. It was completely soluble in water and 1N sodium hydroxide solution. The purified EPS contained 84% of total carbohydrates, 11.2% of reducing sugars, 2% of moisture, 0.8% of proteins and 0.6% uronic acid. The EPS consisted mainly of glucose (91%) with minor quantities of rhamnose and arabinose (1.8% each). Gel permeation chromatography indicated considerable heterogeneity of EPS, having three fractions with molecular weights ranging from 1.0 · 104 to 1.5 · 106 Da. Enzymatic hydrolysis with pullulanase and a-amylase and NMR analysis of the EPS indicated the presence of a (1 fi 6) linkages and the absence of a (1 fi 4) linkages.

Item Type: Article
Uncontrolled Keywords: Dahi; EPS medium; Exopolysaccharide; Fermentation; Leuconostoc
Subjects: 600 Technology > 05 Chemical engineering > 04 Fermentation Technology
600 Technology > 08 Food technology > 27 Dairy products
Divisions: Dept. of Biochemistry
Food Microbiology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 11 Feb 2011 05:56
Last Modified: 27 Aug 2018 04:21
URI: http://ir.cftri.com/id/eprint/9914

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