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Single step purification of lactoperoxidase from whey involving reverse micelles-assisted extraction and its comparison with reverse micellar extraction

Nandini, K. E. and Rastogi, N. K. (2010) Single step purification of lactoperoxidase from whey involving reverse micelles-assisted extraction and its comparison with reverse micellar extraction. Biotechnology Progress, 26 (3). pp. 763-771.

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Official URL: DOI: 10.1002/btpr.383

Abstract

The extraction of lactoperoxidase (EC 1.11.1.7) from whey was studied using single step reverse micelles-assisted extraction and compared with reverse micellar extraction. The reverse micelles-assisted extraction resulted in extraction of contaminating proteins and recovery of lactoperoxidase in the aqueous phase leading to its purification. Reverse micellar extraction at the optimized condition after forward and backward steps resulted in activity recovery of lactoperoxidase and purification factor of the order of 86.60% and 3.25-fold, respectively. Whereas reverse micelles-assisted extraction resulted in higher activity recovery of lactoperoxidase (127.35%) and purification factor (3.39-fold). The sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) profiles also evidenced that higher purification was obtained in reverse micelles-assisted extraction as compared of reverse micellar extracted lactoperoxidase.

Item Type: Article
Uncontrolled Keywords: Downstream processing; Lactoperoxidase; Activity recovery; Extraction efficiency
Subjects: 600 Technology > 08 Food technology > 07 Food Engineering
600 Technology > 08 Food technology > 27 Dairy products
Divisions: Food Engineering
Depositing User: Users 197 not found.
Date Deposited: 19 Jan 2011 07:14
Last Modified: 28 Dec 2016 11:22
URI: http://ir.cftri.com/id/eprint/9852

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