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Characterization of a new acid stable exo-β-1,3-glucanase of Rhizoctonia solani and its action on microbial polysaccharides

Vijayendra, S. V. N. and Yutaka, K. (2009) Characterization of a new acid stable exo-β-1,3-glucanase of Rhizoctonia solani and its action on microbial polysaccharides. International Journal of Biological Macromolecules, 44 (1). pp. 92-97.

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Abstract

A new acid stable exo-β-1,3-glucanase of Rhizoctonia solani purified from a commercial source ‘Kitarase-M’, by a combination of ammonium sulfate precipitation, ion-exchange and gel filtration methods, had specific activity of 0.26 U/mg protein, Km and Vmax values of 0.78 mg/ml and 0.27 mM/min/mg protein, respectively. It had molecular weight of 62 kDa with optimum activity at 40 °C temperature and pH 5.0, with high stability at pH of 3–7. Unique amino acid sequence was found at N-terminal end. The substrate specificity studies confirmed that it is an exo-β-1,3-glucanase. It could hydrolyze curdlan powder to release glucose.

Item Type: Article
Uncontrolled Keywords: Characterization; Curdlan; Exopolysaccharides; Exo-β-1,3-glucanase; Oligosaccharides; Purification; Rhizoctonia solani
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology
Divisions: Food Microbiology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 16 Nov 2010 06:54
Last Modified: 27 Aug 2018 04:21
URI: http://ir.cftri.com/id/eprint/9786

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