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Physico-chemical characterization of a new heteropolysaccharide produced by a native isolate of heterofermentative Lactobacillus sp. CFR-2182

Vijayendra, S. V. N. and Palanivel, E. G. and Mahadevamma, S. and Tharanathan, R. N. (2009) Physico-chemical characterization of a new heteropolysaccharide produced by a native isolate of heterofermentative Lactobacillus sp. CFR-2182. Archives of Microbiology, 191. pp. 303-310.

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Abstract

A heterofermentative Lactobacillus sp. CFR- 2182 was isolated from dahi samples and it was found to produce 8.0 and 20.5 g/L heteropolysaccharide (HePS) in EPS medium (a simplified synthetic medium) and modified MRS broth, respectively, after 72 h at 30�C. The total carbohydrate, reducing sugar and moisture contents of the purified HePS were 74, 10.6 and 2 g, respectively, per 100 g on dry weight basis. The HePS produced in EPS medium had glucose and mannose in 17:1 ratio. The HePS was non-gelling and non-film forming type. It was completely soluble in water and 1 N sodium hydroxide solution. Gel permeation chromatography and HPLC analysis indicated considerable heterogeneity of the HePS, having three fractions with molecular weights ranging from 3.3 9 104 to 1.32 9 106 Da. The enzymatic hydrolysis of the HePS with pullulanase and a-amylase [with a(1?4) linkage] indicated the presence of a(1?6) and traces of a(1?4) linkages, respectively. NMR analysis of the EPS revealed unique chemical shifts.

Item Type: Article
Uncontrolled Keywords: Chromatography � EPS medium � Fermentation � Heteropolysaccharide � Lactobacillus
Subjects: 600 Technology > 05 Chemical engineering > 04 Fermentation Technology
500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 02 Bacteriology
Divisions: Food Microbiology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 16 Nov 2010 06:48
Last Modified: 30 Jul 2012 03:52
URI: http://ir.cftri.com/id/eprint/9785

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