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Hyperhydricity-Related Morphologic and Biochemical Changes in Vanilla (Vanilla planifolia)

Reddampalli, V. Sreedhar and Venkatachalam, Lakshmanan and Bhagyalakshmi, N. (2009) Hyperhydricity-Related Morphologic and Biochemical Changes in Vanilla (Vanilla planifolia). Journal of Plant Growth Regulation, 28. pp. 46-57.

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Abstract

Shoot cultures of vanilla (Vanilla planifolia) showed a progressive change toward hyperhydricity syndrome (HHS) leading to the necrosis of shoot buds when transferred to liquid medium of shake-flask type from solid (gelled) medium (S). HHS was also associated with severe damage at cellular and subcellular levels, an increase in free polyamines (PAs) and accumulation of water, a decrease in quantities of chlorophyll and protein, and drastic changes in reducing and nonreducing sugars. Spermine was by far the major polyamine in all the analyzed cultures. The progression toward and onset of HHS showed higher activities of antioxidant enzymes, indicative of the shoots’ defensive efforts against oxidative stress. The specific enzyme activities of normal and H2 stages were 342.6 and 350.35 U mg-1 protein for peroxidase (POD, EC 1.11.1.11), 38.4 and 30.38 U mg-1 protein for superoxide dismutase (SOD, EC 1.15.1.1), and 71.3 and 82.75 U mg-1 protein for catalase (CAT, EC 1.11.1.6), respectively. The kinetic parameters of the culture medium suggested that nutrient utilization was normal in HHS and that the severe biochemical alterations and cellular damage were mainly due to oxidative stress.

Item Type: Article
Uncontrolled Keywords: Vanilla planifolia � Complete immersion system � Hyperhydricity syndrome � Morphologic changes � Biochemical changes
Subjects: 500 Natural Sciences and Mathematics > 10 Plants > 02 Plant Biochemistry
500 Natural Sciences and Mathematics > 10 Plants
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 15 Nov 2010 07:05
Last Modified: 28 Dec 2011 10:19
URI: http://ir.cftri.com/id/eprint/9781

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