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Purification and Partial Characterization of Intracellular Fructosyltransferase from a Novel Strain of Aureobasidium pullulans

Agbaje, Lateef and Julius Kola, Oloke and Prapulla, S. G. (2007) Purification and Partial Characterization of Intracellular Fructosyltransferase from a Novel Strain of Aureobasidium pullulans. Turkish Journal of Biology, 31. pp. 147-154.

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Abstract

The intracellular fructosyltransferase (FTase) of a novel strain of Aureobasidium pullulans (CFR 77) capable of producing 59% of fructooligosaccharides (FOS) within 9 h of reaction time was obtained by wet-milling, and then purified and characterized. The purified FTase revealed 2 bands of 147 and 170 KD; its activity was optimum at an approximate pH of 5.0 and temperature of 55 °C. The specific activity of the final purified material was 42, representing a purification factor of 79.44 and yield of 43%. The enzyme is very stable, retaining more than 80% of its original activity at the optimum reaction conditions after 12 h. Using the crude intracellular FTase, 59% of FOS was produced within 9 h of reaction time, which is a considerable reduction in the reaction time of 12-25 h that has been reported in the literature. The purified FTase yielded 59% of FOS within 3 h of reaction time.

Item Type: Article
Uncontrolled Keywords: Fructosyltransferase, Aureobasidium pullulans, enzyme purification, fructooligosaccharides
Subjects: 600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes
500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 04 Fungi
Divisions: Fermentation Technology and Bioengineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 28 Sep 2010 11:10
Last Modified: 23 Oct 2018 10:36
URI: http://ir.cftri.com/id/eprint/9743

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