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Degradation of 3-chlorobenzoate and phenol singly and in mixture by a mixed culture of two ortho-pathway-following Pseudomonas strains

Jayachandran, V. P. and Kunhi, A. A. M. (2009) Degradation of 3-chlorobenzoate and phenol singly and in mixture by a mixed culture of two ortho-pathway-following Pseudomonas strains. Journal of Industrial Microbiology Biotechnology, 36. pp. 219-227.

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Abstract

The compatibility and eYciency of two orthocleavage pathway-following pseudomonads viz. the 3-chlorobenzoate (3-CBA)-degrader, Pseudomonas aeruginosa 3mT (3mT) and the phenol-degrader, P. stutzeri SPC-2 (SPC-2) in a mixed culture for the degradation of these substrates singly and simultaneously in mixtures was studied. Another phenol-degrading strain, Pseudomonas sp. SoPC-5 (SoPC-5) that utilizes a meta-cleavage mode also was tried in co-culture with 3mT. The former combination was found to be a better degrader of both the substrates when present alone. But, with inoculum levels of 0.15 mg cell dry wt each of 3mT/SPC-2 or 3mT/SoPC-5 growth with 2 mM each of 3-CBA and phenol was slow with a lag of 24 h and degradation being incomplete. However, with higher inocula in the ratios 1:1, 1:2, and 2:1, i.e., 0.3 + 0.3, 0.3 + 0.6, and 0.6 + 0.3 mg cell dry wt of 3mT and SPC-2, respectively complete degradation of both the substrates occurred. Degradation of 3-CBA was complete with the release of stoichiometric amounts of chloride (Cl¡) when concentrations of phenol/3-CBA were varied as 2:2, 2:4, and 4:2 mM, i.e., even when the concentration of the more toxic co-substrate 3-CBA was higher than phenol eVective simultaneous degradation occurred at the inoculums ratio of 1:1 (0.3 mg dry cell wt. of each strain). These studies clearly indicated the better suitability of ortho-cleavage-utilizing strains as partners in a mixed culture than those follow diVerent modes.

Item Type: Article
Uncontrolled Keywords: 3-Chlorobenzoate Phenol P. aeruginosa · P. stutzeri Mixed culture Simultaneous degradation
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 24 Organic Chemistry
500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology
Divisions: Food Microbiology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 28 Sep 2010 04:57
Last Modified: 28 Dec 2011 10:18
URI: http://ir.cftri.com/id/eprint/9721

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