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Optimization of Synthesis of Ethyl Isovalerate Using Rhizomucor miehei Lipase

Chowdary, G. V. and Prapulla, S. G. (2003) Optimization of Synthesis of Ethyl Isovalerate Using Rhizomucor miehei Lipase. Applied Biochemistry and Microbiology, 39 (3). pp. 243-248.

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Abstract

—Immobilized lipase from Rhizomucor miehei (Lipozyme IM-20) was used to catalyze the esterification reaction between isovaleric acid and ethanol to synthesize ethyl isovalerate in n -hexane. Response surface methodology based on a four-variable, five-level, central composite rotatable design was employed to optimize four important reaction variables—enzyme/substrate (E/S) ratio, substrate concentration, incubation time, and temperature— affecting the synthesis of ethyl isovalerate. The optimum conditions predicted for achieving maximum ester yield (500 mM) are as follows: E/S ratio, 48.41 g/mol; substrate concentration, 1 M; reaction time, 60 h; and temperature, 60° C. The predicted value matched well with the experimentally obtained value of 487 mM.

Item Type: Article
Uncontrolled Keywords: Lipases, Immobilized lipase, fatty acid esters, ethyl isovalerate, immobilized Rhizomucor miehei
Subjects: 600 Technology > 08 Food technology > 01 Analysis
500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 16 Enzyme Chemistry
Divisions: Fermentation Technology and Bioengineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 24 Sep 2010 09:33
Last Modified: 28 Dec 2011 10:18
URI: http://ir.cftri.com/id/eprint/9692

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