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Toxicity and Antibacterial Activity of Carotenoids produced by Aspergillus Carbonarius in Caenorhabditis Elegans as an In Vivo Model

Deepthi, S. (2010) Toxicity and Antibacterial Activity of Carotenoids produced by Aspergillus Carbonarius in Caenorhabditis Elegans as an In Vivo Model. [Student Project Report]

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type: Student Project Report
Additional Information: There is a continuing need for the development of simple animal models for the study of toxicity of pharmaceutical compounds and for the study of host-pathogen interactions. The implementation of non mammalian model organisms such as Drosophila melanogaster and Caenorhabditis elegans has recently proven successful thereby reducing both cost and the ethical constrainsts associated with virulence studies using mammalian hosts. In this present study, the toxicity and antibacterial potential of carotenoids produced by Aspergillus carbonarius were carried out using C. elegans as a model. The carotenoid was found to be non toxic to the worms up to a concentration of 4000 μg / mL. However there was a mild toxicity (1.59 %) observed at a concentration of 6000 μg / mL. Although the carotenoid seems to be non toxic, the worms exposed to the pigment expressed β-galactosidase as a stress response, probably due to poor distribution of oxygen supply by surrounded carotenoids. The well based method for antibacterial assay did not give favorable results since all the worms were died during incubation. Hence a plate based method was developed. In the plate based method, the survival rate of the worms was a dose dependent in carotenoid treated samples. It opens up a new way for screening of compounds which are less soluble in polar solvents like DMSO. From these above results, it is concluded that C. elegans can be a versatile model system to study the biological activities of pharmaceutically important compounds.
Uncontrolled Keywords: Carotenoids; fat-soluble pigments; antimicrobial activity;
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology
Divisions: Food Microbiology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 13 Jul 2010 07:01
Last Modified: 28 Dec 2011 10:16
URI: http://ir.cftri.com/id/eprint/9538

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