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Development and Characterization of Aspergillus carbonarius mutants for Metabolite Production

Shwetha, V. (2010) Development and Characterization of Aspergillus carbonarius mutants for Metabolite Production. [Student Project Report]

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type: Student Project Report
Additional Information: As a group of organisms, the filamentous fungi impact mankind in both very positive negative ways. Yet as a whole they are poorly understood and surprisingly understudied organisms. However, within the genus Aspergillus exist some of the most intensely studied filamentous fungal species. The present work describes the mutant development ( A. carbonarius ). The mutants and sub‐mutants were developed after UV‐treatment. All the mutants selected after mutation were subjected to grow under different cultural condition. “Mutant-3” has shown good growth compared to other muatants. Mutant-1 was superior in enzyme activity whereas, Mutant-4 was superior in carotenoid pigment production under acidic pH (pH -3). So, in order to enhance the metabolites the mutants were further subjected to UV-mutation ( sub-mutation). Mutant-1 has shown 8.4363 EU/ml of enzyme activity (pH -3), when compared to other mutants, which is similar to that of enzyme activity of wild type i.e., 9.0 EU/ml. There is no improvement in the enzyme activity. However, the Mutant-4 has yielded 3.28 μg of pigment/g dcm of pigment when compared to wild type (0.75 μg of pigment/g dcm). There is 237% of improvement in the pigment yield. There is no improvement in enzyme production by submerged fermentation even after sub-mutation. However, solid state fermentation has shown maximum enzyme activity of 13.325 EU/ml (Fig. 12), by sub-mutant ‘TEN’. There is improvement in enzymatic activity when compared to SmF ( 9.0 EU/ml). Thus, There is 48% of improvement in the Enzyme production by SSF. From the above observation we can conclude that, sub-mutation has yielded improvement in polygalaturonase activity but it has no influence on improvement of pigment (carotenoid) production. However, mutation ( Mutant-4) has shown improvement in pigment yield compared to wild strain.
Uncontrolled Keywords: mutant development; Filamentous fungi; Aspergillus; UV irradiation
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 04 Fungi
500 Natural Sciences and Mathematics > 07 Life Sciences > 03 Biochemistry & Molecular Biology
Divisions: Food Microbiology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 13 Jul 2010 05:27
Last Modified: 28 Dec 2011 10:16
URI: http://ir.cftri.com/id/eprint/9537

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