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Isolation, Screening and Mass Culture of High Lipid and Hydrocarbon Yielding Microalgae

Abhishek, V. Naik (2010) Isolation, Screening and Mass Culture of High Lipid and Hydrocarbon Yielding Microalgae. [Student Project Report]

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type: Student Project Report
Additional Information: In the wake of depleting petroleum reservoirs and the rise in green house gas (CO2) levels, it is necessary to find alternative fuel sources. The oil seeds based and lignocelluloses based biofuels such as bioethanol, butanol and biodiesel compete with food crops for land and water resources and wide scale development of biodiesel from these sources cannot realistically satisfy the existing demand for transport fuels, hence algae based oil production gains importance, since they can be grown in lakes and ponds, in sea and in arid regions. They require little nutrient supply, since they are autotrophic. Algal lipids and hydrocarbons are cheap and reliable alternatives for biodiesel production. Algae produce lipids as energy storage molecules during their secondary stage of growth. For the biodiesel production from algae to be economical, algae with high lipid producing capabilities have to be isolated. The selected algae are grown in media and culture conditions which induce high lipid and biomass production. In the current study, different algal species were isolated from water bodies in and around Mysore. The pure cultures were screened for lipid content using Nile red staining method and high lipid producing strains were selected for further studies. The selected algae were grown in media and culture conditions which induce high lipid and biomass production. The selected isolates were subjected to stress conditions such as Nitrogen and Phosphorous depletion since lipid production in algae is known to increase during stress conditions. The isolates were grown in varying concentrations of nitrogen in medium and also different nitrogen sources such as NaNO3, KNO3, Urea, Ammonium carbonate, Ammonium nitrate and Ammonium acetate, the cultures were incubated for 21 days and the biomass was harvested to extract lipids. The extracted lipids were converted to Fatty Acid Methyl Esters (FAMEs) and injected into GC and GCMS for lipid profiling. Mass culture of microalgae is carried out in closed (Bag) reactors and Open (Aquarium tank) ponds. The isolated algae when grown in nitrogen limited condition produce lipid as high as 64% of dry cell weight (DCW) or 1.2g/l of culture medium.
Uncontrolled Keywords: Microalgae, Lipids, Hydrocarbons, Nitrogen stress
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 01 Algae
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 13 Jul 2010 05:04
Last Modified: 28 Dec 2011 10:16
URI: http://ir.cftri.com/id/eprint/9535

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