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Analysis of Rhizospheric Microbial Diversity of Himalayan Soil by A.R.D.R.A

Thejas, M. Ponnappa (2010) Analysis of Rhizospheric Microbial Diversity of Himalayan Soil by A.R.D.R.A. [Student Project Report]

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type: Student Project Report
Additional Information: The project was focused on the study of bacterial strains isolated from the rhizospheric region of Himalayan soil samples. The glycerol stocks of twenty strains were collected from the Department of Food Microbiology, Central Food & Technological Research Institute, Mysore. The strains were subjected to phenotypic, biochemical and molecular characterization. The phenotypic characterization of the 20 bacterial strains was carried out by Gram’s staining. The type (Gram-positive/ Gram-negative) and morphology of the organism was determined microscopically. All the twenty strains were found to be Gram-negative while their morphology varied from cocci to bacilli. The isolated bacterial strains were then subjected to biochemical tests to determine the metabolic activities of the cultures. Carbohydrate utilization tests (for glucose, sucrose, maltose and starch), citrate utilization test, urease test and caseinase tests were carried out. Of the twenty strains, ten were positive for glucose fermentation, six for sucrose fermentation, eight for maltose fermentation and only four for starch hydrolysis. All twenty strains were found to utilize citrate as a sole carbon source. Nine strains were positive for urease test and about twelve strains showed the zone of clearing when caseinase test was conducted. The strains were then subjected to molecular characterization. The DNA of each of the strains was isolated and subjected to amplification by Polymerase Chain Reaction. Restriction digestion was then carried out using the restriction enzyme, Bgl II. The number of fragments and the sizes of the digested samples were analyzed by agarose gel electrophoresis. Based on these findings, the microbial diversity of the strains isolated from the rhizospheric region of Himalayan soil was studied.
Uncontrolled Keywords: soil samples; bacterial strains; biochemical tests; molecular characterization
Subjects: 500 Natural Sciences and Mathematics > 05 Earth Sciences > 02 Soil Sciences
500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology
Divisions: Food Microbiology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 12 Jul 2010 11:35
Last Modified: 28 Dec 2011 10:16
URI: http://ir.cftri.com/id/eprint/9528

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