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Enzymatic Production of N-Acetyl-D-Glucosamine from Chitin

Chaithra, A. Poonacha (2010) Enzymatic Production of N-Acetyl-D-Glucosamine from Chitin. [Student Project Report]

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type: Student Project Report
Additional Information: Production of thermoactive chitinases by local isolates of Aspergillus flavus, Fusarium oxysporum and Penicillium monoverticillium were carried out under solid state fermentation using commercial wheat bran. A. falvus produced maximum 26.37 unit/ g of initial dry substrate (U/g IDS) of endo-chitinase and 222.1 U/g IDS of β-Nacetyl- D-hexosaminidase. F. oxysporum produced 13.28 U/g IDS of endo-chitinase and 168.3 U/g IDS β-N-acetyl-D-hexosaminidase. P. monoverticillium produced 41.0 U/g IDS of endo-chitinase and 195.4 U/g IDS β-N-acetyl-D-hexosaminidase. The crude enzyme showed maximum activity at 62±2oC and pH 4.6 for endochitinase. The chitinases obtained from Aspergillus flavus, Fusarium oxysporum and Penicillium monoverticillium were concentrated by dialysis using high molecular weight polyethylene glycol. The concentrated chitinase systems were effective in hydrolysis of colloidal chitin and production of N-acetyl-D-glucosamine, indicating its potential for industrial applications.
Uncontrolled Keywords: thermoactive chitinases; Solid state fermentation; N-acetyl-D-glucosamine
Subjects: 600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes
Divisions: Meat Fish and Poultry Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 12 Jul 2010 05:46
Last Modified: 28 Dec 2011 10:15
URI: http://ir.cftri.com/id/eprint/9506

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