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Molecular Identification of Marine Proteolytic bacteria

Pathrika, Kuttappa K. (2010) Molecular Identification of Marine Proteolytic bacteria. [Student Project Report]

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type: Student Project Report
Additional Information: Marine microbial enzymes are known to have much higher catalytic activity towards a broad range of substrates. In the present study, marine proteolytic bacteria isolated from marine samples were qualitatively screened on casein agar medium and were characterized by morphological and biochemical analysis. One portion of 16S rRNA genes of selected marine bacteria was amplified by PCR and 16S rDNA of marine bacterial strain 188 M was partially sequenced. The sequence data was analyzed and phylogenic tree of marine bacterial strain 188 M was constructed using online molecular biology tools. The results of this study showed that out of several isolates 26 M, 133 M and 188 M were potent protease producers, which showed conspicuous clearance zone around the colonies in casein agar plates. The PCR amplification of 16S rRNA gene was resulted in the amplification of 1.3 kb long DNA fragment from the respective genomic DNAs. The BLAST analysis of 16S rRNA gene sequence showed that the bacterial strain 188 M is a Bacillus sp, which is very close to Bacillus sp. KJW98 (Acc No Fj716700.1). The phylogenic tree showed the evolutionary relationship of the 188 M with other Bacillus sp.
Uncontrolled Keywords: Marine microbial enzymes; Proteolytic enzymes; Proteases;
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 06 Marine Microbiology
600 Technology > 08 Food technology > 28 Meat, Fish & Poultry
Divisions: Meat Fish and Poultry Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 12 Jul 2010 04:43
Last Modified: 28 Dec 2011 10:15
URI: http://ir.cftri.com/id/eprint/9505

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