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Application of marine bacterial protease for protein hydrolysate preparation from fish processing waste

Deepthi, B.V. (2010) Application of marine bacterial protease for protein hydrolysate preparation from fish processing waste. [Student Project Report]

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type: Student Project Report
Additional Information: Production of protease by marine Staphylococcus sp. 26M, Vibrio sp. 133M and Bacillus sp. 188M under submerged fermentation using shrimp bio-waste was carried. The activity of crude proteases produced by Staphylococcus sp. 26M, Vibrio sp. 133M and Bacillus sp. 188M were 16.2 U/mL, 4.5 U/mL and 6.7 U/mL respectively at 42 h of incubation. The crude proteases produced were characterized for the optimum pH and temperature. The proteases produced by Staphylococcus sp. 26M, Vibrio sp. 133M, Bacillus sp. 188M exhibited their optimum activity at 32±2oC. Similarly, pH 7.6 was the optimum for maximum activity of the proteases by Staphylococcus sp. 26M, Vibrio sp. 133M, Bacillus sp. 188M. The proteases produced were partially purified by ammonium sulphate precipitation followed by dialysis. The maximum activity of purified proteases was 22.8 U/mL, 28.0 U/mL and 20.0 U/mL by Staphylococcus sp. 26M, Vibrio sp. 133M, Bacillus sp. 188M respectively. Finally the partially purified proteases were used for protein hydrolysates preparation from fish processing waste of Sardine (Sardina pilchardus) and Mackerel (Scomber austriasicus). The protein hydrolysates prepared were tested for the antioxidant activity by DPPH scavenging activity method. The protein hydrolysates prepared using the protease of Bacillus sp. 188M has showed more antioxidant activity as compared to the protein hydrolysates prepared using the proteases obtained from Staphylococcus sp. 26M and Vibrio sp. 133M.
Uncontrolled Keywords: Staphylococcus; Shrimp bio-waste; protease; Sardine; Mackerel
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 06 Marine Microbiology
600 Technology > 08 Food technology > 28 Meat, Fish & Poultry
Divisions: Meat Fish and Poultry Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 12 Jul 2010 04:37
Last Modified: 28 Dec 2011 10:15
URI: http://ir.cftri.com/id/eprint/9504

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