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Optimization of β-N-acetylhexosaminidase production under solid state fermentation by Fusarium oxysporum using response surface methodology

Elakkiyaselvi, V. (2010) Optimization of β-N-acetylhexosaminidase production under solid state fermentation by Fusarium oxysporum using response surface methodology. [Student Project Report]

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type: Student Project Report
Additional Information: Production of β-N-acetylhexasosaminidase from Fusarium oxysporum by solid state fermentation (SSF) using commercial wheat bran (CWB) and optimization of fermentation condition by response surface methodology (RSM) were carried out. The optimization of process variables for β-N-acetylhexosaminidase production by F. oxysporum in SSF were carried out by RSM using a 3**(4-1) fractional factorial design comprising 1 block and 27 runs. The design was employed by selecting incubation temperature (X1,ºC), incubation time (X2, h), initial moisture content of the substrate (X3,% w/w) and inoculum level (X4, μl) as experimental factors. The results of design experiments showed that incubation temperature (p≤0.001), incubation time (p≤0.05) and initial moisture content (p≤0.01) have significant effect on β-N-acetylhexosaminidase production. R2 was observed during validation experiment showed the usefulness of the model and the optimum conditions were determined as incubation temperature 26ºC, incubation time 190 hr, initial moisture content of the substrate 69.5 (% w/w) and inoculum level 25 μl/ 5 g substrate. The crude enzyme showed maximum activity at 62±1ºC and pH of 4.6. The molecular weight of the β-N-acetylhexasosaminidase was found to be approximately 94 kDa. The crude enzymes system is effective in hydrolysis of colloidal chitin and production of GlcNAc, showing its potential use in industrial application. MFPT Dept,
Uncontrolled Keywords: commercial wheat bran; Chitin; Solid state fermentation; phytopathogenic fungi
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology
Divisions: Meat Fish and Poultry Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 25 Jun 2010 09:35
Last Modified: 28 Dec 2011 10:14
URI: http://ir.cftri.com/id/eprint/9425

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