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Organogenesis from cotyledon and hypocotyl-derived explants of japhara (Bixa orellana L.)

Parimalan, Rangan and Giridhar, Parvatam and Harischandra, B. Gururaj and Ravishankar, G. A. (2007) Organogenesis from cotyledon and hypocotyl-derived explants of japhara (Bixa orellana L.). Acta Botanica Croatica, 66 (2). pp. 153-160. ISSN 0365–0588

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Abstract

A protocol for direct organogenesis in Bixa orellana (pink flowers variety) has been developed with significant organogenic response from rooted hypocotyls, hypocotyl segments, and cotyledonary leaf explants on Murashige and Skoog (MS) medium supplemented with 2.0 mg L–1 thidiazuron and 0.25% coconut water or 7.0 mg L–1 N6-Benzyladenine and 0.1 mg L–1 a-naphthalene acetic acid. Thidiazuron in combination with coconut water promoted higher organogenic response in rooted hypocotyls. Similarly direct organogenesis was noticed from hypocotyls, cotyledonary leaf explants and shoot tip explants on half MS medium with 0.25 mg L–1 N6-Benzyladenine (BA) and 0.5 mg L–1 indole-3-acetic acid (IAA). Best shoot elongation of shoot buds was achieved in the presence of 1.5 mg L–1 (BA) + 1.0 mg L–1indole-3-butyric acid (IBA). The in vitro rooting of microshoots was good in the presence of 3.0 mg L–1 (IBA). Seventy percent of rooted plants survived after acclimatization.

Item Type: Article
Uncontrolled Keywords: organogenesis, axillary bud, Bixa orellana, hypocotyl, shoot, rooting
Subjects: 500 Natural Sciences and Mathematics > 10 Plants
500 Natural Sciences and Mathematics > 10 Plants > 05 Tissue Culture
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 23 Mar 2010 04:09
Last Modified: 28 Mar 2018 11:49
URI: http://ir.cftri.com/id/eprint/9406

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