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Studies on antioxidants and antimeastatic components from spices against cancer

Harish Nayaka, M. A. (2007) Studies on antioxidants and antimeastatic components from spices against cancer. PhD thesis, University of Mysore.

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Abstract

Cancer is a complex multi-step disease, rising constantly in both developing and developed countries leading to mortality. Developing a strategy for cancer therapy has been difficult since cancer cell poses lots of challenges to normal cells by self-regulating their survival and dysregulating normal cellular action. Antioxidants, quenchers of free radicals - that initiates cancer, chemo/radio therapeutics, that inhibit proliferative cancer cell, surgery/immuno therapies that envisage the removal of the affected tissue/cell, have been in practice for cancer therapy; yet cancer cells find their way successful due the limitations encountered in these therapies such as lack of discrimination between cancer cell and a normal cell. Current thesis entitled “Studies on Antioxidants and Antimetastatic Components from Spices Against Cancer” explores the possibility of dietary antioxidants and antimetastatic polysaccharides that can effectively bring down oxidative stress and target galectin-3, a key molecule in metastasis causing severity of cancer pathogenicity and death as revealed in scheme - 1. During the study, potential water soluble antioxidants and antimetastatic polysaccharides from Black cumin – BC - Nigella Sativa and Swallow root – SR- Decalepis hamiltonii have been identified, characterized and determined their efficacy in vitro and in vivo. Results indicated tannic acid and gentisic acid followed by gallic, protocatechuic, and vanillic acid as major multi-potent antioxidants in BC and SR respectively. SR also found to contain higher levels of 2-Hydroxy-4-methoxybenzaldehyde (HMBA) but contributing little to the activity. A potent galectin-3 inhibitory pectic polysaccharide was also identified in SR - SRPP and structure – function analysis reveal the presence of higher levels of galactose and arabinose and their probable contribution to antimetastatic potency. Antioxidants of BC AAb bbs sst ttr rra aac cct tt vii and SR and antimetastatic SRPP was effective in inhibiting Reactive oxygen species - mediated hepatotoxicity/cervical cancer and B16F10 melanoma cells induced lung metastasis in vivo, suggesting their in vivo efficacy against cancer. Overall data thus suggest that selected sources have both antioxidants and antimetastatic components that can effectively inhibit cancer spread/metastasis.

Item Type: Thesis (PhD)
Uncontrolled Keywords: Black cumin-; Swallow root-; Antioxidants-; Isolation and characterization
Subjects: 600 Technology > 08 Food technology > 30 Spices/Condiments
600 Technology > 01 Medical sciences > 09 Human Physiology
600 Technology > 08 Food technology > 32 Antioxidants
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 15 Mar 2010 05:36
Last Modified: 15 Mar 2010 05:36
URI: http://ir.cftri.com/id/eprint/9394

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