[feed] Atom [feed] RSS 1.0 [feed] RSS 2.0

Porcine pancreatic alpha amylase and its isoforms—Effect of deglycosylation by peptide-N-glycosidase F.

Anitha, Gopal B. and Sridevi Annapurna, Singh and Muralikrishna, G. (2008) Porcine pancreatic alpha amylase and its isoforms—Effect of deglycosylation by peptide-N-glycosidase F. International Journal of Biological Macromolecules, 43 (2). pp. 100-105. ISSN 0141-8130

[img] PDF
International_Journal_of_Biological_Macromolecules_43_2_2008_p_100-105.pdf
Restricted to Registered users only

Download (331kB)
Official URL: http://dx.doi.org/10.1016/j.ijbiomac.2008.03.008

Abstract

Porcine pancreatic α-amylase (PPA) and its isoforms (PPA-I and PPA-II) were deglycosylated by peptide-N-glycosidase F (PNGase F) to investigate the role of bound carbohydrate. On deglycosylation, the effect on thermal stability was less pronounced. Deglycosylation resulted in a shift of the mid-point of thermal transition by 1–2 °C towards lower temperature. The fluorescence emission maxima of PPA, PPA-I and PPA-II were found to be 340 nm indicating the presence of tryptophan residues in a fairly hydrophilic environment. A red shift in emission spectra accompanied by an increase in fluorescence intensity was observed upon deglycosylation.

Item Type: Article
Uncontrolled Keywords: Glycoprotein; Peptide-N-glycosidase F; Thermal stability; Spectral studies
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 13 Carbohydrate Chemistry
500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 25 Peptide Chemistry
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 09 Mar 2010 06:15
Last Modified: 01 Jul 2015 12:02
URI: http://ir.cftri.com/id/eprint/9375

Actions (login required)

View Item View Item