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Simple HPLC Method for Resolution of Curcuminoids with Antioxidant Potential

Madhava Naidu, M. and Shyamala, B. N. and Manjunatha, J. R. and Sulochanamma, G. (2009) Simple HPLC Method for Resolution of Curcuminoids with Antioxidant Potential. Journal of Food Science, 74 (4). c312-c318.

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Development of methodology for qualitative analysis and quantitative separation of individual curcuminoids in curcumin removed turmeric oleoresin (CRTO) and study of antioxidant activity of individual curcuminoids have been achieved in the present study. A simple HPLC protocol was outlined for optimal resolution of curcuminoids (I), (II), and (III) on an Exil-NH2 column using isocratic elution with a mixture of isopropanol and water. CRTO is shown to be good source for the compounds II and III, as they are in an enriched form. The optimum resolution of the curcumin and its analogs in the present method developed, affords a facile method for estimation of individual curcuminoids in turmeric samples, which is an improvement over the standard AOAC method which estimates only total curcuminoids. The free radical scavenging capacity of pure curcuminoids I, II, and III, as determined by DPPH method at 100 ppm concentration was 88%, 80%, and 68% with IC50 values being 56, 62, and 73 ppm, respectively. Their antioxidant potential could render them useful as important nutraceuticals or functional food ingredients.

Item Type: Article
Uncontrolled Keywords: antioxidant potential, CRTO, curcuminoids, HPLC analysis, thin layer chromatography, turmeric
Subjects: 600 Technology > 08 Food technology > 30 Spices/Condiments
600 Technology > 08 Food technology > 32 Antioxidants
Divisions: Plantation Products Spices and Flavour Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 26 Feb 2010 11:25
Last Modified: 21 Dec 2016 12:51
URI: http://ir.cftri.com/id/eprint/9369

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