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Production of alpha-amylase under solid-state fermentation utilizing coffee waste

Pushpa, S. Murthy and Madhava Naidu, M. and Srinivas, Pullabhatla (2009) Production of alpha-amylase under solid-state fermentation utilizing coffee waste. Journal of Chemical Technology and Biotechnology, 84. pp. 1246-1249.

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Coffee industry substrates such as coffee pulp, coffee cherry husk, silver skin, spent coffee andmixtures of these coffee wastes (MC) were evaluated for their efficacy as sole carbon source for the synthesis of α-amylase in solid-state fermentation (SSF) using a fungal strain of Neurospora crassa CFR 308. For SSF with coffee pulp and with MC, α-amylase activity of 3908 U g−1 ds (units per gram of dry substrate) and 3870 U g−1 ds, respectively, was observed. Parameters such as moisture (60%), pH (4.6), temperature (28 ◦C), particle size (1.0mm), inoculum size (107 spores g−1 ds), and fermentation time (5 days) were optimized for enzyme synthesis,wherein 4981 and 4324 U g−1 Ug−1 ds of α-amylase activity was obtained in SSF with coffee pulp andMC, respectively. The enzyme production was further improved when the substrates were subjected to pre-treatment by steaming. Accordingly,maximumα-amylase activity of 7084 U g−1 ds and 6342 U g−1 ds was obtained with steam-pretreated coffee pulp andMC, respectively, demonstrating them to be excellent sole carbon sources for synthesis of α-amylase production.

Item Type: Article
Uncontrolled Keywords: Neurospora crassa CFR 308; α-amylase; solid-state fermentation; coffee pulp; coffee wastes
Subjects: 600 Technology > 05 Chemical engineering > 04 Fermentation Technology
600 Technology > 07 Beverage Technology > 04 Coffee
Divisions: Plantation Products Spices and Flavour Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 26 Feb 2010 10:20
Last Modified: 19 Jul 2012 04:19
URI: http://ir.cftri.com/id/eprint/9361

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