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Reverse micellar extraction for downstream processing of lipase: Effect of various parameters on extraction

Nandini, K. E. and Navin, K. Rastogi (2009) Reverse micellar extraction for downstream processing of lipase: Effect of various parameters on extraction. Process Biochemistry, 44. pp. 1172-1178.

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Abstract

Reversemicellar extraction of lipase using cationic surfactant cetyltrimethylammonium bromide (CTAB) was investigated. The effect of various process parameters on both forward and backward extraction of lipase from crude extract was studied to optimize its yield and purity. Forward extraction of lipase was found to be maximum using Tris buffer at pH 9.0 containing 0.10 M NaCl in aqueous phase and 0.20 M CTAB in organic phase consisting of isooctane, butanol and hexanol. In case of backward extraction, lipase was extracted from the organic phase to a fresh aqueous phase in 0.05 M potassium phosphate buffer (pH 7.0) containing 1.0 MKCl. The activity recovery, extraction efficiency and purification factor of lipase were found to be 82.72%, 40.27% and 4.09-fold, respectively. The studies also indicated that the organic phase recovered after back extraction could be reused for the extraction of lipase from crude extract.

Item Type: Article
Uncontrolled Keywords: Reverse micellar extraction Downstream processing Lipase Cationic surfactant Activity recovery Extraction efficiency
Subjects: 600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes
Divisions: Food Engineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 25 Feb 2010 10:31
Last Modified: 28 Dec 2016 11:23
URI: http://ir.cftri.com/id/eprint/9337

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