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Production and characterization of bacterial polyhydroxyalkanoate copolymers and evaluation of their blends by fourier transform infrared spectroscopy and scanning electron microscopy

Shamala, T. R. and Divyashree, M. S. and Reeta, Davis and Latha Kumari, K. S. (2009) Production and characterization of bacterial polyhydroxyalkanoate copolymers and evaluation of their blends by fourier transform infrared spectroscopy and scanning electron microscopy. Indian Journal of Microbiology, 49. pp. 251-258.

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Abstract

Rhizobium meliloti produced a copolymer of short chain length polyhydroxyalkanoate (scl-PHA) on sucrose and rice bran oil as carbon substrates. Recombinant Escherichia coli (JC7623ABC1J4), bearing PHA synthesis genes, was used to synthesize short chain length-co-medium chain length PHA (scl-co-mcl-PHA) on glucose and decanoic acid. Fourier transform infrared spectroscopy (FTIR) spectra of the PHAs indicated strong characteristic bands at 1282, 1723, and 2934 cm–1 for scl-PHA and at 2933 and 2976 cm–1 for scl-co-mcl-PHA polymer. Differentiation of polyhydroxybutyrate (PHB) and polyhydroxybutyrate- co-hydroxyvalerate-P(HB-co-HV) copolymer was obseverd using FTIR, with absorption bands at 1723 and 1281 for PHB, and at 1738, 1134, 1215 cm–1 for HV-copolymer. The copolymers were analyzed by GC and 1H NMR spectroscopy. Films of polymer blends of PHA produced by R. meliloti and recombinant E. coli were prepared using glycerol, polyethylene glycol, polyvinyl acetate, individually (1:1 ratio), to modify the mechanical properties of the fi lms and these fi lms were evaluated by FTIR and scanning electron microscopy.

Item Type: Article
Uncontrolled Keywords: Polyhydroxyalkanoate · Fourier transform infrared spectroscopy · PHA blends · Rhizobium meliloti · Recombinant Escherichia coli · Scanning electron microscopy
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 10 Spectroscopic and Spectrometric analysis
500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 02 Bacteriology
Divisions: Food Microbiology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 25 Feb 2010 05:31
Last Modified: 31 Jul 2012 11:01
URI: http://ir.cftri.com/id/eprint/9329

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