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Altering functional attributes of proteins through cross linking by transglutaminase – A case study with whey and seed proteins

Anuradha, S. N. and Prakash, V. (2009) Altering functional attributes of proteins through cross linking by transglutaminase – A case study with whey and seed proteins. Food Research International, 42. pp. 1259-1265.

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Abstract

The effect of cross linking of the major whey protein b-lactoglobulin (b-Lg) and major protein fractions (11S) of soybean (Glycinin) and sesame seed (a-globulin) with the microbial enzyme transglutaminase (EC 2.3.2.13) was studied. The formation of polymerized proteins was followed by poly acrylamide gel electrophoresis, gel filtration high pressure liquid chromatography (HPLC) and evaluation of functional properties. Cross linked proteins were less turbid on heating to higher temperature as compared to untreated samples and the temperature at which the protein turns turbid also increased in the treated samples. In case of b-Lg and a-globulin of sesame seed when the control showed turbidity at 60 �C, the enzyme treated sample indicated at 65 �C and higher. Similar results were obtained in the case of soybean also. The treated samples showed higher emulsifying activity when compared to the control. The control showed an emulsifying activity of 0.55 ± 0.02, and the treated sample showed an emulsifying activity of 0.72 ± 0.02 (optical density at 500 nm is taken as emulsifying activity). Foaming capacity did not improve significantly with the enzyme treatment. The complex formed was investigated by gel filtration chromatography. Nearly 30% of the proteins (11S protein fractions and b-Lg) formed the complex and increase in the concentration of the proteins or the enzyme did not show any increase in the complex formation. The changes in the fluorescence intensity indicated changes in the microenvironment of the chromophores induced by the enzymatic cross linking.

Item Type: Article
Uncontrolled Keywords: Soybean Sesame seed Bovine milk whey Transglutaminase Emulsifying activity Foaming capacity Cross linking
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 29 Protein Chemistry
600 Technology > 08 Food technology > 27 Dairy products
600 Technology > 08 Food technology > 19 Lipids-oils/fats > 01 Oilseeds
Divisions: Protein Chemistry and Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 24 Feb 2010 09:04
Last Modified: 26 Apr 2012 06:15
URI: http://ir.cftri.com/id/eprint/9315

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