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Aqueous Two Phase Extraction for Purification and Concentration of Biomolecules from Pineapple (Ananas comosus L. Merryl)

Ravindra Babu, B. (2008) Aqueous Two Phase Extraction for Purification and Concentration of Biomolecules from Pineapple (Ananas comosus L. Merryl). PhD thesis, University of Mysore.

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Abstract

Thesis mainly covers the separation, purification and concentration of biomolecules by aqueous two phase extraction and membrane processes. Standardization of process parameters to obtain optimal separation during aqueous two phase extraction and membrane processes is carried out. A practical approach for simultaneous fractionation and purification of mixture of enzymes (bromelain and polyphenol oxidase) from pineapple (Ananas comosus L. Merryl) by aqueous two phase extraction is developed. Major emphasis is given to develop alternative/newer approaches for the concentration of biomolecules and liquid foods. Performance evaluation of direct osmosis process for the concentration of biomolecules (bromelain enzyme from pineapple waste) and liquid foods (pineapple juice) is carried out in detail. Modeling of mass and heat transfer in osmotic membrane distillation process is studied in detail. The basic study of mathematical modeling of the osmotic membrane distillation/direct osmosis enhances the understanding of the process. Also, contribution of concentration and temperature polarization effects during osmotic membrane distillation process is discussed. Integrated approaches for the purification and concentration of biomolecules are studied in detail. The proposed protocols will be helpful in developing effective and efficient processes for the purification and/or concentration of enzymes, proteins and liquid foods. The major aspects covered in the thesis are the development of improved/newer processes for the purification and concentration of biomolecules and liquid foods. xvii

Item Type: Thesis (PhD)
Uncontrolled Keywords: aqueous two phase extraction membrane processes pineapple purification biomolecules liquid foods
Subjects: 600 Technology > 08 Food technology > 24 Fruits
600 Technology > 08 Food technology > 05 Processing and Engineering
Divisions: Food Engineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 06 Oct 2009 10:54
Last Modified: 06 Oct 2009 10:54
URI: http://ir.cftri.com/id/eprint/9277

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