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Extraction, Purification and Concentration of Canthaxanthin from Aspergillus Carbonarius

Krupa, D. (2009) Extraction, Purification and Concentration of Canthaxanthin from Aspergillus Carbonarius. [Student Project Report]

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type: Student Project Report
Additional Information: A mutant strain of Aspergillus carbonarius produces canthaxanthin (~574 Da) during submerged fermentation. The pigment was extracted from dried biomass using four organic solvents (methanol, ethanol, acetone and hexane) of varying polarity and the extracts were processed using three different nanofiltration (NF-200, NF-250 and GKSS) and one nonporous (NP-NTGS2200) membranes. Fine fraction of biomass (75-105 μm) resulted in greater extractability of canthaxanthin (2.26 mg/g of biomass) compared to all other size fractions in ethanol. Among the four solvents, hexane extract exhibited the highest extractability of canthaxanthin (5.83 mg/g of dried biomass) with a purity of 32 mg/g of total soluble solids. The overall extraction performance was 6-19 fold higher in hexane compared to polar solvents. Even the level of impurities, namely, carbohydrates, salts and acids coextracted with hexane was also much lower. During membrane processing, NF–GKSS membrane with methanol extract exhibited greater rejection (89%), recovery (84%) and enrichment factor (4.19 fold) of canthaxanthin among all the combinations of membrane and solvent extracts studied. However, the overall membrane performance taking in to consideration of flux was greater with NF-250 with ethanol extract followed by NF-200 with methanol extract. The membrane selectivity towards canthaxanthin with respect to other impurities especially nonpolar impurities needs further investigation. These results suggested that two solvent systems, extraction with a nonpolar solvent such as hexane followed by membrane processing in a polar solvent medium would improve the overall extractability, purity and recovery of canthaxanthin.
Uncontrolled Keywords: Aspergillus carbonarius canthaxanthin Extraction Purification Estimation
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 04 Fungi
500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 14 Carotenoid Chemistry
Divisions: Food Engineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 26 Sep 2009 07:04
Last Modified: 28 Dec 2011 10:12
URI: http://ir.cftri.com/id/eprint/9222

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