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Physical Chemistry of Aqueous Two Phase System and Partitioning of Biomolecule

Prakruthi, H.R. (2009) Physical Chemistry of Aqueous Two Phase System and Partitioning of Biomolecule. [Student Project Report]

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type: Student Project Report
Additional Information: ATPS have been extensively used for the recovery and purification of many biomolecules. To study the partitioning behavior of the protein, effect of PEG molecular weight, phase composition and protein concentration was evaluated. PEG / Ammonium sulphate system, with three different molecular weights of PEG (4000, 6000, and 8000) at three different phase composition was studied. Physical properties of the phase system (Density, Viscosity, Interfacial tension) were measured. Purification of ADH from Beaker yeast was carried out using PEG/MDX polymer/ polymer system. Effect of TLL and Volume ratio was studied. At 46.58% TLL with Volume ratio 0.33, 6 fold purification was observed.
Uncontrolled Keywords: Aqueous two phase systems Physical properties
Subjects: 600 Technology > 08 Food technology > 14 Physical properties
600 Technology > 08 Food technology > 05 Processing and Engineering
Divisions: Food Engineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 17 Sep 2009 11:23
Last Modified: 28 Dec 2011 10:11
URI: http://ir.cftri.com/id/eprint/9174

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