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Lipase-Catalyzed Synthesis of Isoamyl Butyrate: Optimization by Response Surface Methodology

Hari Krishna, S. and Manohar, B. and Divakar, S. and Karanth, N. G. (1999) Lipase-Catalyzed Synthesis of Isoamyl Butyrate: Optimization by Response Surface Methodology. Journal of the American Oil Chemists' Society, 76 (12). pp. 1483-1488.

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Abstract

Immobilized lipase from Mucor miehei (Lipozyme IM-20) was employed in the esterification of butyric acid and isoamyl alcohol to synthesize isoamyl butyrate in n-hexane. Response surface methodology based on five-level, five-variable central composite rotatable design was used to evaluate the effects of important variables—enzyme/substrate (E/S) ratio (5–25 g/mol), acid concentration (0.2–1.0 M), alcohol concentration (0.25–1.25 M), incubation period (12–60 h), and temperature (30–50°C)—on esterification yield of isoamyl butyrate. In the range of parameters studied, the extent of esterification decreased with temperature, lower E/S ratios, and incubation periods. Excess acid and alcohol concentrations (i.e., acid/alcohol > 1.4 or alcohol/acid > 1.4) were found to decrease yield probably owing to inhibition of the enzyme by acid or alcohol, the former being more severe. The optimal conditions achieved are as follows: E/S ratio, 17 g/mol; acid concentration, 1.0 M; incubation period, 60 h; alcohol concentration, 1.25 M; and temperature, 30°C. With these conditions, the predicted value was 1.0 M ester, and the actual experimental value was 0.98 M.drawback. Considering the high demand and benefits, an optimized process with high yields for the economic enzymatic synthesis of isoamyl butyrate is important. The goal of the present investigation was to optimize the process for enzymatic synthesis of isoamyl butyrate using response surface methodology employing a five-level, five-variable central composite rotatable design (CCRD). The variables affecting the esterification that were considered were enzyme/ substrate (E/S) ratio, acid concentration, incubation period, alcohol concentration, and reaction temperature.

Item Type: Article
Uncontrolled Keywords: Central composite rotatable design, enzymatic synthesis, esterification, isoamyl butyrate, lipase, Lipozyme IM-20, Mucor miehei, response surface methodology.
Subjects: 600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes
600 Technology > 08 Food technology > 05 Processing and Engineering
Divisions: Fermentation Technology and Bioengineering
Food Engineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 01 Jul 2009 05:33
Last Modified: 01 Oct 2018 10:51
URI: http://ir.cftri.com/id/eprint/9139

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