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Biotransformation of codeine to morphine in freely suspended cells and immobilized cultures of Spirulina platensis

Ramachandra Rao, S. and Usha, Tripathi and Ravishankar, G. A. (1999) Biotransformation of codeine to morphine in freely suspended cells and immobilized cultures of Spirulina platensis. World Journal of Microbiology and Biotechnology, 15. pp. 465-469.

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Abstract

Both freely suspended cells and immobilized cultures of Spirulina platensis, a blue-green alga, biotransformed exogenously fed codeine, an opium alkaloid, to morphine. The external addition of codeine to the culture medium did not a�ect the growth of S. platensis. Immobilization of Spirulina in a calcium alginate gel matrix was optimized by using 2% (w/v) sodium alginate and reducing the concentration of nutrients of Zarrouk's medium, which caused destabilization of the calcium alginate gel. The accumulation of morphine increased gradually and reached maxima of 330 lg 100 ml)1 culture at 105 h in freely suspended and 351 lg 100 ml)1 at 96 h in immobilized Spirulina cultures. Accumulation of morphine was detected only in the medium, whereas cells did not show accumulation. The immobilized Spirulina cultures showed marginally higher conversion of codeine to morphine over freely sus- pended cultures.

Item Type: Article
Uncontrolled Keywords: Biotransformation, codeine, immobilization, morphine, Spirulina platensis
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology > 01 Algae
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 30 Jun 2009 09:48
Last Modified: 28 Dec 2011 10:10
URI: http://ir.cftri.com/id/eprint/9133

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