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Electrophoretic Extraction of Cells/Particles in a Counter Current Extractor

Chethana, S. and Ganapathi, Patil and Madhusudhan, M. C. and Raghavarao, K. S. M. S. (2008) Electrophoretic Extraction of Cells/Particles in a Counter Current Extractor. Separation Science and Technology, 43. pp. 3583-3600.

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Abstract

Electrophoretic separation is a leading method for resolving mixtures of charged macromolecules or cells. In the present work, a combination of free electrophoresis and multistage extraction called Electrophoretic Counter Current Extractor was explored. A simple mathematical model was developed to describe the mass and heat transfer during the electrophoretic separation. In order to validate the model and also to test the extractor, the extraction of fixed human Red Blood Cells and Latex particles of different sizes suspended in phosphate buffer, was performed at different electric field strengths. The experimental results were found to agree reasonably well with those predicted by the model.

Item Type: Article
Uncontrolled Keywords: Counter current, electrophoresis, electrophoretic mobility, extraction, separation
Subjects: 600 Technology > 08 Food technology > 05 Processing and Engineering
Divisions: Food Engineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 16 Jun 2009 10:23
Last Modified: 16 Jun 2009 10:23
URI: http://ir.cftri.com/id/eprint/9094

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