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Supercritical carbon dioxide extraction of xanthones with antioxidant activity from Garcinia mangostana: Characterization by HPLC/LC-ESI-MS

Zarena, A. S. and Udaya Sankar, K. (2009) Supercritical carbon dioxide extraction of xanthones with antioxidant activity from Garcinia mangostana: Characterization by HPLC/LC-ESI-MS. The Journal of Supercritical Fluids, 49 (3). pp. 330-337. ISSN 0896-8446

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Abstract

The xanthones from mangosteen (Garcinia mangostana) pericarp was extracted with and without alcohol as entrainer at 30MPa and 50C in supercritical carbon dioxide (SC-CO2). It was found that entrainer increases the rate of extraction and yield substantially. SC-CO2+ethanol (4%) at 20MPa, 40C was investigated by HPLC/LC-ESI-MS for characterization of the xanthones. From liquid chromatography electron spray ionization mass spectrometry (LC-ESI-MS) spectra, 12 components were structurally correlated and confirmed based on the fragmentation characteristics and information available in the literature. The occurrence of these xanthone in SC-CO2 extract is reported for first time in G. mangostana species. The antioxidant activities of SC-CO2 were investigated by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radicals (IC50=41.8[mu]g/mL), lipid peroxidation (IC50=21.5[mu]g/mL), hydroxyl radicals site specific (IC50=30.0[mu]g/mL) and hydroxyl radicals non-site specific (IC50=23.1[mu]g/mL). From cytochrome c assay it was observed that SC-CO2 extract can reduce ferric cytochrome c to ferrocytochrome c. The results showed that SC-CO2 had great potential as antioxidant agent.

Item Type: Article
Uncontrolled Keywords: Supercritical carbon dioxide Mangosteen Xanthones HPLC LC-MS Antioxidant activity
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 06 Chromatography
600 Technology > 08 Food technology > 24 Fruits
600 Technology > 08 Food technology > 32 Antioxidants
Divisions: Food Engineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 23 Feb 2018 08:31
Last Modified: 23 Feb 2018 08:31
URI: http://ir.cftri.com/id/eprint/9087

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