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Enzyme-linked immunoassay for the detection of glyphosate in food samples using avian antibodies

Arul Selvi, A. and Sreenivas, M. A. and Manonmani, H. K. (2011) Enzyme-linked immunoassay for the detection of glyphosate in food samples using avian antibodies. Food and Agricultural Immunology, I. pp. 1-12.

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Abstract

A simple competitive immunoassay was developed for the measurement of glyphosate in food samples. It employed the avian antibodies (IgY) that recognised glyphosate as a capture reagent and glyphosate�alkaline phosphatase conjugate as an enzyme label. The assay depended on the competitive binding between the antibody and glyphosate derived from food samples for binding sites with immobilised glyphosate�OVA conjugate. The concentration of glyphosate in the food samples was quantified by the ability of the pesticide present in food samples to inhibit the binding of the enzyme conjugate to the antibody and subsequently the colour formation in the assay. The assay was specific to glyphosate with a limit of detection of 2 ppb. Mean analytical recovery of glyphosate in different food samples was 9.00�134.00%. The precision of the assay was satisfactory. The assay compared favourably with high-pressure liquid chromatography (HPLC) in its ability to accurately measure glyphosate in the food samples.

Item Type: Article
Uncontrolled Keywords: glyphosate; avian antibodies (IgY); enzyme-linked immunosorbent assays; herbicide; food samples; high-pressure liquid chromatography; immunology; hapten
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 26 Pesticide Chemistry
600 Technology > 08 Food technology > 18 Processed foods
Divisions: Dept. of Biochemistry
Fermentation Technology and Bioengineering
Food Safety Analytical Quality Control Lab
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 15 Jun 2009 07:15
Last Modified: 15 May 2012 11:05
URI: http://ir.cftri.com/id/eprint/9066

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