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Characterization of acid-induced molten globule like state of ficin

Devaraj, K. B. and Kumar, Parigi Ramesh and Prakash, V. (2009) Characterization of acid-induced molten globule like state of ficin. International Journal of Biological Macromolecules, 45. pp. 248-254. ISSN 0141-8130

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Abstract

Effect of pH on the conformational behavior of ficin (EC 3.4.22.3), a cysteine protease from the latex of Ficus carica was monitored by circular dichroism, fluorescence spectroscopy, ANS binding and hydrodynamic studies. The results obtained from near- and far-UV CD, intrinsic fluorescence and ANS binding studies demonstrate that ficin exhibits the characteristic properties of molten globule at acidic conditions between the pH 1.2 and 2.0. Ficin at pH 1.4 retained about ~ 74% secondary structure with a substantial loss of tertiary structure. The acid-induced state was found to have a compact shape as measured by stokes radius on size-exclusion chromatography.

Item Type: Article
Uncontrolled Keywords: Ficin Protein folding Molten globule Acid-unfolding Cysteine protease
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 29 Protein Chemistry
Divisions: Protein Chemistry and Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 27 Jun 2017 06:47
Last Modified: 27 Jun 2017 06:47
URI: http://ir.cftri.com/id/eprint/9034

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