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Value addition to corncob: Production and characterization of xylooligosaccharides from alkali pretreated lignin-saccharide complex using Aspergillus oryzae MTCC 5154

Ayyappan, Appukuttan Aachary and Prapulla, S. G. (2009) Value addition to corncob: Production and characterization of xylooligosaccharides from alkali pretreated lignin-saccharide complex using Aspergillus oryzae MTCC 5154. Bioresource Technology, 100 (2). pp. 991-995. ISSN 0960-8524

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Abstract

Comparison of various pretreatments such as mild alkali/acid treatments and pressure cooking of corncob to expose its lignin-saccharide complex has been carried out to enhance enzymatic hydrolysis of xylan to xylooligosaccharides (XOS). Scanning electron micrographs of lignin-saccharide complex of native and pretreated corncob powder showed that the complex was greatly altered during alkali pretreatment. Hydrolysis of alkali pretreated corncob powder using a commercial endoxylanase produced 811.5% of XOS in the hydrolyzate equivalent to 5.80.14mgml-1 of XOS. Reaction parameters for the production of XOS from corncob using endoxylanase from Aspergillus oryzae MTCC 5154 were optimized and an XOS yield of 10.20.14mgml-1 corresponding to 813.9% with 73.5% xylobiose was obtained. HPLC/RID and ESI/MS analysis of XOS mixture and purified fractions showed that XOS was a mixture of neutral oligosaccharides of DP, 2-7.

Item Type: Article
Uncontrolled Keywords: Xylooligosaccharides (XOS) Aspergillus oryzae MTCC 5154 Corncob Lignin-saccharide complex Endoxylanase
Subjects: 600 Technology > 08 Food technology > 16 Nutritive value > 05 Enzymes
600 Technology > 08 Food technology > 22 Legumes-Pulses
Divisions: Fermentation Technology and Bioengineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 15 Jun 2009 05:34
Last Modified: 21 Jun 2012 06:14
URI: http://ir.cftri.com/id/eprint/9026

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