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Characterization of Acidic Proteases from Poultry Intestine

Cicily, Shiny (2009) Characterization of Acidic Proteases from Poultry Intestine. [Student Project Report]

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type: Student Project Report
Additional Information: A study was conducted on the application of ammonium sulphate precipitation technique for purification of acidic proteases from chicken intestine. Acid protease can be obtained from chicken intestine by applying simple and effective method of ammonium sulphate precipitation. The sample was subjected to different saturation from 40%-80% and the protein fractions were collected at each level. Highest purification was obtained in 40% saturation, which partially purified enzyme that can be used in tannery industry, fermentation industry, meat tenderization, baking industry and in diary industry. Characterization of partially purified acidic protease showed optimum pH of 4, temperature optimum of 400C, which showed stability even after incubating for 90 minutes. The activity can be enhanced using metal ions such as ZnSO4, CaCl2, and MgCl2. Molecular weight of the partially purified enzyme ranged from 27KD- 97KD. Proteases make up to 48% of industrial enzymes and are mostly used in detergents, leather production and food industry. Therefore extraction and purification of acidic protease is necessary which not only retains the valuable biomolecules but also avoid pollution of environment that is caused due to the byproducts obtained from poultry industries.
Uncontrolled Keywords: Acid protease chicken intestine ammonium sulphate precipitation
Subjects: 600 Technology > 08 Food technology > 28 Meat, Fish & Poultry
Divisions: Meat Fish and Poultry Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 03 Jun 2009 07:23
Last Modified: 28 Dec 2011 10:08
URI: http://ir.cftri.com/id/eprint/8973

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