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Incorporation of long chain n-3 PUFA into rice bran oil by enzymatic acidolysis

Rajni, Chopra and Sambaiah, K. (2009) Incorporation of long chain n-3 PUFA into rice bran oil by enzymatic acidolysis. Journal of Food Science and Technology, 46 (1). pp. 26-30.

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The production of structured lipids by acidolysis of rice bran oil (RBO) with long chain n-3 polyunsaturated fatty acids (PUFA) in hexane was carried out using immobilized lipase from Rhizomucor miehei. Long chain n-3 PUFA obtained after hydrolysis of cod liver oil, were used as acyl donors. The study of complex interaction among incubation time and temperature, substrate to molar ratio and enzyme concentration and identification of the optimal combination have been carried out using central composite rotatable design (CCRD) experiments. The experimental data was fitted to a response surface method based on the CCRD, and linear relationship was observed between the predicted and observed values. The optimum conditions generated from the models indicated that maximum incorporation of eicosapentaenoic acid and docosahexaenoic acid were ~ 12% at 1:10 molar ratio of RBO: long chain n-3 PUFA, when incubation was carried out for 26 h at 40°C and enzyme concentration was 5.5% of total substrates weight. Incubation time and temperature did not affect the incorporation significantly whereas incorporation of long chain n-3 PUFA into RBO was significantly increased with increase in enzyme concentration and substrate molar ratio.

Item Type: Article
Uncontrolled Keywords: Rice bran oil, Cod liver oil, Long chain n-3 PUFA, Acidolysis, Lipase
Subjects: 500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 17 Fatty Acid Chemistry
600 Technology > 08 Food technology > 19 Lipids-oils/fats
Divisions: Dept. of Biochemistry
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 11 Mar 2009 05:23
Last Modified: 28 Dec 2011 10:07
URI: http://ir.cftri.com/id/eprint/8890

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