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Optimization of polyhydroxybutyrate production by β-carotene producing strain of Micrococcus sp.

Vijayendra, S. V. N. and Veeramani, S. and Shamala, T.R (2008) Optimization of polyhydroxybutyrate production by β-carotene producing strain of Micrococcus sp. Journal of Food Science and Technology, 45 (6). pp. 506-509.

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Abstract

Optimization of the polyhydroxybutyrate (PHB) production by a pigmented Micrococcus sp. was done by using glucose, sucrose, lactose and fructose as carbon sources, initial pH between 6.0 and 8.0, fermentation temperature of 30 and 37°C, inoculum of 5, 10 and 15% v/v and by submerged/aerobic fermentation. Maximum production of polyhydroxyalkanoates (PHA) was at 37°C in sucrose containing pH 6.0 under aerobic conditions. Overall, the PHB production ranged between 35 and 40% of the dry cell weight. In GC analysis, PHA and PHB produced the same peak. The UV-visible spectrophotometer and HPLC analysis of the pigment indicated presence of (β-carotenoids with absorption maxima at 454 and 348 nm.

Item Type: Article
Uncontrolled Keywords: Micrococcus, Poly-β-hydroxyalkanoates, Optimization, β-Carotenoids, Fermentation
Subjects: 500 Natural Sciences and Mathematics > 07 Life Sciences > 04 Microbiology
Divisions: Food Microbiology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 11 Mar 2009 04:18
Last Modified: 27 Aug 2018 04:18
URI: http://ir.cftri.com/id/eprint/8888

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