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Preferential extractability of γ -oryzanol from dried soapstock using different solvents

Raj, R Kumar and Purnima Kaul, Tiku and Prakash, V. (2009) Preferential extractability of γ -oryzanol from dried soapstock using different solvents. Journal of Science of Food and Agriculture, 89. pp. 195-200.

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Abstract

BACKGROUND: γ -Oryzanol from rice bran has lately gained potential importance because of its proven health benefits. Thus the extractability of γ -oryzanol from the soapstock of crude rice bran oil is important from the perspective of future large-scale production, which would give value addition to this by-product obtained from the rice bran oil industry. The aim of the present study was to investigate the extraction of γ -oryzanol from the drum-dried soapstock of rice bran oil using various solvents. RESULTS: Itwasfoundthat γ -oryzanol couldbe extractedmost effectively usingethyl acetate, followedbydichloromethaneand ethyl methyl ketone. All components of γ -oryzanol have an alcohol group in the ferulate portion giving rise to relatively high polarity, thereby increasing the extraction in more polar solvents efficiently. Ethyl acetate showed maximum extractability of γ -oryzanol by the Soxhletmethod. To quantify γ -oryzanol, reverse phase high-performance liquid chromatography (RP-HPLC) was used for fingerprinting the γ -oryzanol analogues with respect to standard γ -oryzanol. CONCLUSION: A new RP-HPLC method for determining the individual components of γ -oryzanol has been reported that can be used for performing an online characterisation of γ -oryzanol analogues by liquid chromatography/mass spectrometry.

Item Type: Article
Uncontrolled Keywords: extractability; hydrophobicity; γ -oryzanol; RP-HPLC; soapstock
Subjects: 600 Technology > 08 Food technology > 21 Cereals > 01 Rice
500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 06 Chromatography
Divisions: Protein Chemistry and Technology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 05 Jan 2009 10:56
Last Modified: 28 Dec 2011 10:07
URI: http://ir.cftri.com/id/eprint/8876

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