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Extraction of bovine serum albumin using nanoparticulate reverse micelles

Umesh Hebbar, H. and Raghavarao, K.S.M.S. (2007) Extraction of bovine serum albumin using nanoparticulate reverse micelles. Process Biochemistry, 42 (12). pp. 1602-1608.

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Abstract

The extraction of a relatively large molecular weight protein, bovine serum albumin (BSA), using nano-sized reverse micelles of nonionic surfactant polyoxyethylene p-t-octylphenol (Triton-X-100) is attempted for the first time. Suitability of reverse micelles of anionic surfactant sodium bis (2-ethyl hexyl) sulfosuccinate (AOT) and Triton-X-100/AOT mixture in organic solvent toluene for BSA extraction is also investigated. Although, the size of the Triton-X-100 reverse micelle in toluene is large enough to host BSA molecule in the hydraulic core, the overall extraction efficiency is found to be low, which may be due to lack of strong driving force. AOT/toluene system resulted in complete forward extraction at aqueous pH 5.5 and a surfactant concentration of 160 mM. The back extraction with aqueous phase (pH 5.5) resulted in 100% extraction of BSA from the organic phase. The addition of Triton-X-100 to AOT reduced the extraction efficiency of AOT reverse micelles, which may be attributed to reduced hydrophobic interaction. The circular dichroism (CD) spectrum of BSA extracted using AOT/toluene reverse micelles indicated the structural stability of the protein extracted.

Item Type: Article
Uncontrolled Keywords: Anionic; AOT; BSA; Nonionic; Reverse micelle; Surfactant; Toluene; Triton-X-100
Subjects: 600 Technology > 08 Food technology > 16 Nutritive value > 03 Proteins
500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 09 Quantitative Analysis
Divisions: Food Engineering
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 19 Aug 2008 07:16
Last Modified: 28 Dec 2011 10:05
URI: http://ir.cftri.com/id/eprint/8726

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