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Biochemical Studies On Capsaicin Synthase

Siri, C.G. (2005) Biochemical Studies On Capsaicin Synthase. [Student Project Report]

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Abstract

This Dissertation / Report is the outcome of investigation carried out by the creator(s) / author(s) at the department/division of Central Food Technological Research Institute (CFTRI), Mysore mentioned below in this page.

Item Type: Student Project Report
Additional Information: <p align="justify">Capsaicin synthase is the key regulatory enzyme in capsaicin biosynthesis. So far, there are no reports on purification and characterization of this enzyme. The present work was investigated with a purpose of partial purification and characterization of capsaicin synthase from placental tissues of Capsicum annuum var Arka Lohit from IIHR, Bangalore. Capsaicin synthase has been purified to near electrophoretic homogeneity, which was found to be heterodimer with a molecular weight of 53 kDa and 35 kDa. Using different chromatographic techniques, two dimers were separated in active form. 53 kDa subunit was found to be 8 methyl nonanoic acid dehydrogenase activity which biosynthesizes 8-methyl-nonenoic acid whereas 35 kDa subunit is capsaicin synthase. Purified capsaicin synthase was characterized for optimum substrate concentration in terms of km and v-max values. It was also found that capsaicin synthase is a cationic protein as confirmed by DEAE cellulose.</p>
Uncontrolled Keywords: capsaicin synthase capsaicin biochemical studies
Subjects: 600 Technology > 08 Food technology > 23 Vegetables
600 Technology > 05 Chemical engineering > 03 Enzyme Biotechnology and Engineering
500 Natural Sciences and Mathematics > 04 Chemistry and Allied Sciences > 16 Enzyme Chemistry
Divisions: Plant Cell Biotechnology
Depositing User: Food Sci. & Technol. Information Services
Date Deposited: 23 Jun 2005
Last Modified: 28 Dec 2011 09:24
URI: http://ir.cftri.com/id/eprint/87

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